NGS data analysis

Next-generation sequencing (NGS) technology can generate various types of sequencing data, including whole genome sequencing, whole exome sequencing, RNAseq-seq, ChIP-seq, Methyl-Seq, and many more. But while each of these NGS data types is tailored to specific biological questions, the initial stages of data processing are remarkably consistent across all types. In these common preprocessing steps, data quality is assessed, adapters and low-quality reads are trimmed, duplicates are removed, and data is typically aligned or mapped to a reference genome. Once the data is prepared, the analysis branches into specialized procedures unique to each application. 

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In this section, you will find some content on general NGS preprocessing, as well as subsequent analytical steps specific to different NGS data types.

Preprocessing

Gene Expression

Variant Calling

ChIP-Seq

Single Cell Gene Expression